Kitui is accessible by road from Nairobi through Thika-Matuu road, about 10km; and from Nairobi through Thika to Mwingi, about 210km. Travellers can also travel from Nairobi through the 160km Nairobi-Machakos-Kitui road.
Travellers coming from Mombasa can get to Kitui through Kibwezi. The Mombasa-Nairobi highway is a 310km road to Kibwezi and a further 200km drive from Kibwezi to Kitui town.
Kitui County has 3 airstrips: Ithookwe, Tseikuru and Mutomo airstrips, of which only one is operational (Ithookwe). An airstrip is proposed to be constructed in Mwingi because of the coal mining activities set to begin in Mui basin.